PURIFICATION OF HEMOGLOBIN

摘要

This invention relates to methods for the production of pure hemoglobin solutions, particularly pure hemoglobin solutions derived from expression of hemoglobin in recombinant systems, and compositions thereof. Methods are also disclosed for removal of contaminant hemoglobins using rapid heating to result in substantially protoporphyrin IX-free hemoglobin solutions, and for removal of high amounts of bacterial proteins and simultaneous purification of crude hemoglobin-oontaining starting materials to result in partially purified hemoglobin solutions. Methods for the further purification of partially purified hemoglobin solution and the removal of metal contamination in hemoglobin solutions, if required, are also disclosed.