发明名称 |
DNA amplification and subtraction techniques |
摘要 |
A method of isolating genomic or RNA-derived duplex fragments which are unique to one of two fragment mixtures. The fragments in positive-source and negative-source mixtures are separately equipped with end linkers, and each mixture is amplified by successive primed-strand replications, using a single primer which is homologous to the associated linker. The second-source linker is biotinylated, and the fragments in this mixture are hybridized in molar excess with the fragments in the positive-source mixture. DNA species which are not hybridized with the biotinylated species, i.e., species that are unique to the positive-source mixture, are isolated after removal of hybridized species by affinity chromatography. Also disclosed is a method of amplifying a mixture of DNA fragments by repeated linker/primer replication.
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申请公布号 |
US6107023(A) |
申请公布日期 |
2000.08.22 |
申请号 |
US19880208512 |
申请日期 |
1988.06.17 |
申请人 |
GENELABS TECHNOLOGIES, INC. |
发明人 |
REYES, GREGORY R.;KIM, JUNGSUH |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C07H21/02;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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