ENZYME-LINKED IMMUNO-MAGNETIC ELECTROCHEMICAL BIOSENSOR

摘要

A electrochemical biosensor system based on enzyme-linked immuno-magnetic sandwich assay wherein an interdigitated array of electrodes is equipped wit h a magnet to attract magnetic beads. Magnetic particles bear a first recognition molecule capable of binding to the analyte. Enzymes are chemical ly modified to complex with the analyte. When the sandwich assay is performed, a substrate is added. The substrate is chosen such that it is cleavable by the enzyme in a reporting molecule capable of redox recycling. A substrate when cleaved preferably leads to a p-aminophenol.