| 摘要 |
The present invention provides array compositions comprising a substrate wit h a surface comprising discrete sites, at least one fiducial, and a population of microspheres comprising at least a first and a second subpopulation, each comprising a bioactive agent, and distributed on said surface. Each subpopulation may optionally comprise a unique optical signature, an identifier binding ligand, or both. The invention further provides compositions comprising a computer readable memory, comprising data acquisition, registration, and comparison modules to direct a computer to function in a specified manner. The registration module may utilize any numb er of fiducials, including a fiducial fiber, a fiducial microsphere, or a fiducial template generated from the random array. In further aspects, the invention provided methods of making the array compositions of the invention , for comparing separate data images of a random array, for decoding a random array composition, and for determining the presence of a target analyte in a sample.
|
