Verfahren zur Herstellung eines rekombinanten Adeno-assoziierten Virus, geeignete Mittel hierzu sowie Verwendung zur Herstellung eines Arzneimittels

摘要

The invention relates to a method of producing a recombinant adeno-associated virus (rAAV). According to the inventive method, a helper construct and a vector construct are introduced into a suitable cell at different times. The helper construct contains no nucleic acid sequences, especially except for the AAV promoters, to which at least one rep protein can substantially specifically bind. The vector construct preferably contains ITR sequences in flop orientation. The recombinant adeno-associated viruses produced according to the inventive method are especially useful for producing a tumor cell into which additionally nucleic acids encoding GM-CSF and B7.2 were introduced. Said tumor cell can in turn be used in the form of a medicament for the treatment of cancer.