Disclosed in this invention is a process for preparing an established avian embryonic germ cell line comprising the steps of: (a) culturing primordial germ cells (PGCs) isolated from an avian embryonic gonad in a medium supplemented with a cell growth factor and a differentiation inhibitory factor to obtain EG cell colonies; (b) culturing the EG cells in the same medium as in step (a) by employing a feeder layer until the EG cells are colonized; and (c) recovering and subculturing the EG cells in the same medium as in step (a) to establish the EG cell line.
申请公布号
WO0047717(A1)
申请公布日期
2000.08.17
申请号
WO2000KR00104
申请日期
2000.02.11
申请人
HANMI PHARM. CO., LTD.;HAN, JAE, YONG;PARK, TAE, SUB