METHOD FOR PREPARING VACCINIA VIRUS OF HEPATITIS A

摘要

PURPOSE: A method for preparing vaccinia virus of hepatitis A is provided for simplifying steps of preparation of a large scale of virus antigen using a suspension media which does not contain Ca ion and glutamine. CONSTITUTION: A method for preparing vaccinia virus comprises: inactivating and proliferating by a cell culture of virus; recovering a virus from the proliferated cell; concentrating and then purifying the recovered virus by concentration gradient-ultracentrifugation using solution for concentration gradient which is harmless to human; and preparing the vaccinia virus of hepatitis A by performing dialysis to remove the residual solution.