| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new 'type-10' phosphodiesterase which has a specific amino acid sequence, has a cyclic nucleotide-hydrolyzing activity, and is useful for the study on the mechanism of intracellular propagation of information, the development of a new medicine by the screening of an inhibitor, and so on. SOLUTION: This is a new 'type-10' phosphodiesterase which has an amino acid sequence shown by formula I or II, or an amino acid sequence obtained by deleting, substituting, adding, or inserting one or more amino acid(s) from, in, to, or into the amino acid sequence, has a cyclic nucleotide-hydrolyzing activity, and is useful for the study on the complex mechanism of intracellular propagation of information, the development of a new medicine by screening an inhibitor having high specificity to the enzyme, and so on. This enzyme was obtained by screening EST database using a catalytic region of human phosphodiesterase 5A as a query sequence, followed by expressing a gene obtained by carrying out PCR using the obtained new sequences as primers. |
