| 摘要 |
PROBLEM TO BE SOLVED: To test the susceptibility of cancer cells to carcinostatics in order to judge the resistance ability of the cancer cells to the carcinostatics by detecting the expression amount of a gene in heat shock protein 75 (mt HSP 75) localized in mitochondria. SOLUTION: The susceptibility of cancer cells to carcinostatics is tested by detecting the expression amount of a gene in heat shock protein 75 (mt HSP 75) localized in mitochondria by the use of northern plotting for the transcriptional product of the gene in mt HSP 75 or western plotting for the translation product of the gene in mt HSP 75, because the expression amount of the gene in mt HSP 75 increases in the cancer cells which show resistance to carcinostatics, e.g. cisplatin and the like, and is on a low level in the cancer cells which show susceptibility to carcinostatics. Further, by this susceptibility test for carcinostatics, it is possible to previously judge whether the cancer cells have resistance ability to carcinostatics or not and more effectively carry out treatment. |
