| 摘要 |
A method for producing mevinolin (lovastatin) by a microorganism in a fermentation process having a seed culture stage and a main fermentation stage, including, cultivating a microorganism biomass in the seed culture stage to produce an inoculum, transferring the inoculum into a fermentation medium in the main fermentation stage, and, maintaining steady stage conditions in the main fermentation stage, thereby producing a fermentation broth containing mevinolin. Preferably, the steady state conditions are maintained in the main fermentation stage by one or more of feeding of organic carbon sources, controlling glucose and/or total reducing sugar content, feeding of organic nitrogen sources, controlling pH, controlling foam level, controlling the mass of the fermentation broth by withdrawals and feedings, and, controlling the dissolved oxygen level. |
