| 摘要 |
<p>A method and apparatus for autofocus on a target layer contained within a microplate well is provided. The instrument is capable of optically sensing a reference point on the underside of a microplate (55). This reference point is then used to focus light (11) onto a target layer within the microplate well, the target layer having a location that is in defined relation to the reference point. The reference point is either a surface (53) of the bottom of the microplate well or is an optically detectable mark (154) on the underside of the microplate (55). In an alternate embodiment, a light position sensitive detector (140) is used to enable deterministic autofocus for a plurality of wells on a microplate (55).</p> |
