A method of comparing genomic DNA from two individuals who share a phenotype, cutting the DNA into fragments long enough to contain on average one or several polymorphisms; combining the fragments under hybridisation conditions and recovering mismatch-free heterohybrids, wherein adapters resistant to nuclease digestion are ligated to the ends of the genomic fragments. A related method uses pooled genomic DNA from individuals with a common phenotype. Another related method uses restricted nucleic acid fragments likely to contain on average less than one natural polymorphism.
申请公布号
WO0046402(A1)
申请公布日期
2000.08.10
申请号
WO2000GB00346
申请日期
2000.02.04
申请人
AMERSHAM PHARMACIA BIOTECH UK LIMITED;FIRTH, GREG;ODEDRA, RAJESH, MURU