发明名称 |
Method for increasing hemoprotein production in filamentous fungi |
摘要 |
The present invention relates to methods of producing hemoproteins comprising (a) introducing into a filamentous fungal cell, which is capable of producing the hemoprotein, (i) one or more first control sequences capable of directing the expression of a heme biosynthetic enzyme encoded by a first nucleic acid sequence endogenous to the filamentous fungal cell, wherein the one or more of the first control sequences are operably linked to the first nucleic acid sequence; and/or (ii) one or more copies of one or more second nucleic acid sequences encoding a heme biosynthetic enzyme; (b) cultivating the filamentous fungal cell in a nutrient medium suitable for production of the hemoprotein and the heme biosynthetic enzymes; and (c) recovering the hemoprotein from the nutrient medium of the filamentous fungal cell.
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申请公布号 |
US6100057(A) |
申请公布日期 |
2000.08.08 |
申请号 |
US19970871267 |
申请日期 |
1997.06.09 |
申请人 |
NOVO NORDISK BIOTECH, INC. |
发明人 |
ELROD, SUSAN L.;CHERRY, JOEL R.;JONES, AUBREY |
分类号 |
C12N15/09;C07K14/795;C12N1/15;C12N9/02;C12N9/08;C12N9/10;C12N9/88;C12N15/31;C12N15/80;C12P21/02;C12R1/69;(IPC1-7):C12P21/06;C12N1/14;C07K14/805 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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