| 摘要 |
A method and assay device for performing displacement assays having increased sensitivity. In particular, the assay device includes a first solid support having reversibly immobilised thereon a binding partner having specific binding activity for an analyte of interest, the binding partner capable of being displaced from the first solid support in the presence of the analyte of interest and forming a binding partner/analyte complex; a second solid support bearing a capture moiety, the capture moiety having a specific binding affinity for the binding partner which is greater than that of the analyte of interest for the binding partner, such that the binding partner may be captured by the capture moiety and the analyte of interest displaced from the binding partner/analyte complex; means for conducting the binding partner/analyte complex from the first solid support to the second solid support; and a means for conducting the displaced analyte from the second solid support to the first solid support. |
