MICROORGANISM PRODUCING 5'-INOSINIC ACID AND MANUFACTURING METHOD THEREOF

摘要

PURPOSE: A method and a microorganism, especially an azaserine-resistant mutant of Brevibacterium ammoniagenes CS1019753(KFCC-10814) which produces 5'-inosinic acid are provided to obtain high concentration and high yield of 5'-inosinic acid. A method is characterized by that very little other by-product and impurity exist in the fermentation medium by directly fermenting Brevibacterium ammoniagenes CIAZ109(KFCC-11068). CONSTITUTION: Brevibacterium ammoniagenes CIAZ109(KFCC-11068) which is a mutant strain of Brevibacterium sp. requires adenine and biotin as viable conditions, is sensitive to lysozymes, is viable at over 3500 microg/mL of 3.4-dihydroproline and has resistance against 100 microg/mL of azaserine. A method for producing comprises: conferring azaserine-resistance to the parent strain, Brevibacterium ammoniagenese CS1019753(KFCC-10814) by mutagenesis; culturing and screening the resultant Brevibacterium ammoniagenes CIAZ109(KFCC-11068); comparing azaserine concentration, which Brevibacterium ammoniagenes CIAZ109 resists against, with the parent strain; and producing the 5'-inosinic acid by directly fermenting the mutant in the fermentation medium containing azaserine.