CAPSID PROTEIN OF HUMAN PAPILLOMAVIRUS 18 TYPE AND PSEUDO HUMAN PAPILLOMAVIRUS 18 TYPE

摘要

PURPOSE: Provided is a recombinant plasmid, pAc/18L1-L2, which simultaneously expresses L1 and L2 genes of capsid proteins of Human Papillomavirus(HPV) 18 type in an insect cell. Also, a recombinant baculovirus pAc/18L1-L2 including this recombinant plasmid is provided, which produces Human Papillomavirus 18 type in a large quantity to help the development of preventive vaccine against Human Papillomavirus 18 type. CONSTITUTION: A method for preparation of pseudo Human Papillomavirus 18 type is composed of the following steps of: preparing a recombinant expression vector pAc/18L1-L2 which produces capsid proteins, L1 and L2 of HPV 18 type; transforming Sf-21 insect cells with the recombinant plasmid and baculovirus DNA to prepare a recombinant baculovirus pAc/18L1-L2(KCTC 0414BP); culturing and separating it to obtain Human Papillomavirus 18 type like particle.