BASE SEQUENCE OF PBLUESCRIPT-MDF

摘要

PURPOSE: The base sequence of a gene related to cell degeneration of mammals is provided, which is useful in figuring out the causes of breast cancer and diagnosing the disease more correctly. CONSTITUTION: The base sequence of a gene related to cell degeneration is obtained by the following steps of: i) preparing mammary gland cells; ii) obtaining total RNA from the degenerated cells; iii) making cDNA library; iv) performing mRNA differential display PCR to identify kinds of mRNAs which are differently expressed during the degeneration of mammary gland cells; and v) analyzing base sequences through Northern blot to identify expression pattern of genes; and vi) performing TA-cloning with PCR products using pUC19 vector and pGEM-T vector and sequencing cloned cDNA fragments which are as follows; Mis-1, Mis-2, Mis-3 and Mis-4.