| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject substance very low in cytotoxicity, safe, highly active and useful for an anti-HIV agent, anti-HIV food, etc., by subjecting plant of perilla order to extraction treatment by using water, purifying the extract through a DEAE anion ion-exchange chromatography and collecting the eluted fractions. SOLUTION: This anti-HIV active substance is obtained by cutting, slitting and powdering the leaves, stems, seeds, etc., of the plants belonging to plant of perilla order, preferably, Prostrate Bugle subfamily, Lavender subfamily, Perilla subfamily and Isodon subfamily of plant of perilla family (e.g. blue chirimen perilla, red chirimen perilla, Prunella Spike, Kassou) subjecting the powdered material to extraction treatment by using water at room temperatures to the boiling temperature, adding a precipitant (e.g. cetyl pyridinium chloride) to the extract to form the precipitate, eluting water-soluble components from the precipitate, purifying the eluted material by a DEAE anion ion-exchange chromatography and collecting the fractions eluted by using e.g. a solution of sodium chloride as an eluting liquid. |
