摘要 |
<p>A liquid medium for nerve cells which contains the culture supernatant of primary astroglia cells obtained by incubating the astroglia cells in a nutritional medium containing insulin, transferrin, selenious acid or its salt, progesterone and albumin together with either superoxide dismutase and catalase or alpha -tocopherols. By using the liquid medium, central nerve cells can be stably incubated. Thus, in low density incubation, synapses excellent in the elongation of axons can be quickly formed, while, in high density incubation, it is possible to provide cells forming a nervous network and being excellent in the stability over a prolonged storage.</p> |