| 摘要 |
<p>The present invention relates to a method of identifying a base at a target position in a sample nucleic acid sequence wherein a primer, which hybridises to the sample nucleic acid immediately adjacent to the target position, is provided and the sample nucleic acid and primer are subjected to a polymerase reaction in the presence of a nucleotide whereby the nucleotide will only become incorporated if it is complementary to the base in the target positon, and said incorporation is detected, characterised in that, a single-stranded nucleic acid binding protein is included in the polymerase reaction step.</p> |
