RECOMBINANT PLASMID DNA PSA V27 ENCODING SYNTHESIS OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII AND BACTERIAL STRAIN ESCHERICHIA COLI AS PRODUCER OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII

摘要

molecular biology, microbiological industry, medicinal biotechnology, genetic engineering. SUBSTANCE: invention proposes recombinant plasmid DNA pSA V27 encoding synthesis of soluble streptavidine secreted into periplasm and used as the base for construction of the strain Escherichia coli VKPM B-7758 as a producer of streptavidine. Strain accumulates 180-240 mg/l of streptavidine in cultural fluid for 1-14 h of fermentation. EFFECT: recombinant plasmid indicated above, increased yield of streptavidine. 3 cl, 3 dwg, 4 ex