| 摘要 |
A method for direct radioimmunoassay of antigens or their associated antibodies utilizing a coated test well comprising adding a serum to be tested for an antigen to test apparatus coated with its antibody; incubating the tubes for from 0.5 to 42 hours; aspirating the contents and washing the same with a Tris-HCl and sodium azide mixture; adding purified I-125 labeled antibody into the tube and incubating for from 1 to 24 hours; aspirating and washing the contents; and counting the tube for I-125 radiation. A similar assay for the antibody may be conducted utilizing an antigen coated tube and I-125 labeled antigen. The method for coating these tubes comprises adding the antigen or antibody solution in a Tris-HCl, sodium azide solution into a suitable test tube or well, incubating the tubes at room temperature from 6 to 72 hours, aspirating and washing the contents and storing at from between 2 and 25 DEG C. until use.
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