摘要 |
PURPOSE: A purification method resulting in a material of high purity by using a minimal number of steps, minimizing the use of caustic or toxic materials such as phenol, and increasing the yield of material are provided. CONSTITUTION: A method for purifying a polysaccharide from group B β-hemolytic Streptococcus (GBS) bacteria includes contacting a bacterial fermentation stock with a hydrophobic interaction chromatography (HIC) resin. Additional steps can include a phenol/saline extraction and an ion exchange chromatography. The method results in a product having very high purity. The product of the purification provides a composition which is highly useful in both research and therapeutic settings.
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