PRODUCTION OF MULTIMERIC PROTEIN BY CELL FUSION METHOD

摘要

PURPOSE: A method of producing a multimeric protein from a hybrid cell formed from the fusion of two or more cells, each of which cell is engineered to express one component of the multimeric protein is provided which are widely applicable to the production of proteins having two or more components. CONSTITUTION: A method for producing a multi-component protein comprises: (a) introducing a first nucleotide sequence into a first cell, wherein the first nucleotide sequence encodes a first component of the multi-component protein; (b) introducing a second nucleotide sequence into a second cell, wherein the second nucleotide sequence encodes a second component of the multi-component protein; (c) optionally, repeating step(b) for each remaining component of the multi-component protein; and (d) fusing cells produced from steps (a)-(c) to form a hybrid cell, wherein the hybrid cell expresses the multi-component protein.