| 摘要 |
PURPOSE: A method of quantitative assay for 1,5-anhydroglucitol(AG) and reagent for such quantitative assay are provided to accomplish a simple method of measuring 1,5-AG by substantially and completely removing or eliminating the glucose contained in a specimen. CONSTITUTION: A method of quantitative assay for 1,5-anhydroglucitol(AG) and reagent comprises the steps of converting a glucose into fructose-1,6-diphosphate, allowing an enzyme system for converting 1,5-AG into 1,5-AG-6-phosphate to contact the specimen, dehydrogenizing the 1,5-AG-6-phosphate contained in the specimen by an action of 1,5-AG-6-phosphate dehydrogenase in the presence of the oxidized coenzyme, and measuring the amount of the resultant reduced coenzyme formed by the dehydrogenation.
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