| 摘要 |
<p>Disclosed is a recombinant enzyme which can convert cephalosprin C into glutaryl-7-aminocephalosporanic acid in a bioreactor at a high yield. A bacterial hemoglobin (Vitreoscilla hemoglobin) gene and a D-amino acid oxidase gene are fused to each other by PCR and the fused DNA fragment is cloned and expressed in E. coli. In a bioreactor, the recombiant enzyme VHb-DAAO can sufficiently supply oxygen as an electron acceptor by virtue of the fused hemoglobin, thereby showing an excellent capability of converting cephalosporin C.</p> |
