| 摘要 |
PCT No. PCT/EP98/02785 Sec. 371 Date May 17, 1999 Sec. 102(e) Date May 17, 1999 PCT Filed May 1, 1998 PCT Pub. No. WO99/02691 PCT Pub. Date Jan. 21, 1999The present invention has for an object a koji mold which expresses at least 2 times more endo- and exo-peptidases than the wild type strain of Aspergillus oryzae CNCM I-1882, and especially at least 30 mU of endopeptidase activity, at least 30 mU of leucine-amino-peptidase and at least 10 mU of prolyldipeptidyl-peptidase activity per ml of supernatant when grown in a minimal medium containing 0.2% soy bean proteins. The invention also provides a DNA-binding protein of Aspergillus oryzae (AREA) having at least the amino-acid sequence from amino-acid 1 to amino-acid 731 of SEQ ID NO:2 or functional derivatives thereof. The invention also provides a DNA molecule that comprises an areA gene encoding the DNA-binding protein according to the invention. In a fourth aspect, the invention provides a method for over-producing proteolytic enzymes, comprising cultivating a koji mold according to the invention in a suitable growth medium under conditions where the mold expresses enzymes, and optionally isolating the enzymes in the form of a concentrate. In another aspect, the invention provides the a method for hydrolyzing protein-containing materials using the koji mold of the invention. In a last further aspect, the invention provides a food product comprising a protein hydrolysate obtainable by fermentation with a koji mold of the invention of a material comprising proteins and at least 5 mM of L-glutamine.
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