发明名称 |
Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector |
摘要 |
A process is disclosed for preparing a protein by a eukaryote transformed by multicopy integration of an expression vector into the genome of a yeast, such as Saccharomyces, Hansenula, and Kluyveromyces, or of a mould such as Aspergillus, Rbizopus and Trichoderma, the expression vector containing both an "expressible gene" encoding the protein and a so-called "deficient selection marker meeded for the growth of the yeast or mould in a specific medium", such as the LEU2d, TRP1d or URA3d gene, in combination witha ribosomal DNA sequence, resulting in stable high copy integration of 100-300 copies per cell. This multicopy integration results in an increased production of the desired protein, which can be guar alpha -galactosidase, an oxidase or a hydrolytic enzyme such as a lipase.
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申请公布号 |
US6090574(A) |
申请公布日期 |
2000.07.18 |
申请号 |
US19940364010 |
申请日期 |
1994.12.27 |
申请人 |
UNILEVER PATENT HOLDINGS, B.V. |
发明人 |
GIUSEPPIN, MARCO F.;LOPES, MARIA T. S.;PLANTA, ROELF J.;VERBAKEL, JOHANNES M. A.;VERRIPS, CORNELIS T. |
分类号 |
C12N15/09;C12N1/15;C12N9/20;C12N9/40;C12N15/81;C12P21/02;C12R1/645;(IPC1-7):C12P21/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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