发明名称 |
FLUORESCENT INTENSITY ASSAY FOR PROTEIN OR PEPTIDE BINDING TO NUCLEIC ACIDS |
摘要 |
A method for assaying binding between a fluorophore-labeled compound and an unlabeled compound is provided. The method includes detecting a quenching effect on fluorescence emitted by the fluorophore-labeled compound resulting from binding. The binding is specific and other than nucleobase to nucleobas e. The method is conducted without separating complexes of the fluorophore- labeled compound and the unlabeled compound from the fluorophore-labeled compound prior to quenching effect detecting, and without providing a signal quenching agent to quench fluorescent light. Preferably, the fluorophore- labeled compound is a nucleic acid and the unlabeled compound is a protein. The method can be used for a variety of applications, including screening fo r drug candidates having optimum binding properties, and quantifying the bindi ng affinity of DNA binding proteins for nucleic acids.
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申请公布号 |
CA2358065(A1) |
申请公布日期 |
2000.07.13 |
申请号 |
CA19992358065 |
申请日期 |
1999.12.29 |
申请人 |
INGENEUS CORP. |
发明人 |
ERIKSON, GLEN H.;DAKSIS, JASMINE I. |
分类号 |
G01N21/64;C12N15/09;C12Q1/68;G01N21/78;G01N33/53;G01N33/533;G01N33/542;G01N33/566;G01N33/68;(IPC1-7):C12Q1/68;G01N33/58 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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