| 摘要 |
This invention relates to a method for monitoring a chemical reaction of pharmaceutical relevance, comprising the steps of: consecutively obtaining Raman spectrum samples of a solution of chemical constituents contained with in a reaction vessel, by irradiating, with substantially monochromatic radiatio n, said solution and detecting the scattered radiation; processing said Raman spectrum samples by means of a multivariate data analysis (MVDA) for consecutively generating at least a first main component related to a latent variable indicative of the progress of the reaction wherein the multivariate data analysis is independent from calibration using reference measurements o f objects having a known composition; and determining the extent of the chemic al reaction on basis of said at least a first main component and optionally on basis of additionally one or more main components generated from one or more previously run reactions of the same kind.
|
