COMPOSITIONS AND METHODS FOR STABILIZING THIOL-CONTAINING BIOMOLECULES

摘要

The present invention relates to compositions that are useful for reducing undesirable disulfide bonds in biological molecules. Disclosed herein are compositions that are useful for reactivating oxidized enzymes to restore native activity, as well as compositions that are useful for maintaining thiol compounds such as enzymes in a reduced state. The compositions comprise at least 3 reducing agents with a difference of at least 5 % more or less than the rest nearest agent. Exemplary of the three agents one n-acetyl-1-cysteine, dithiothreitol and methimazole prolonged liquid storage.