发明名称 |
Synthesis and recovery of aspartame involving enzymatic deformylation step |
摘要 |
The invention relates to the synthesis of aspartame involving enzymatic deformylation of an N-formyl- alpha -L-aspartyl-L-phenylalanine compound by treatment with an enzyme having formylmethionyl peptide deformylase activity and having as a co-factor group 5 to 11 bivalent metal ions. The invention also relates to selective preparation and recovery of aspartame from a mixture of N-formyl- alpha - and beta -L-aspartyl-L-phenylalanine compounds by treatment with such enzyme. And finally, the invention relates to one-pot enzymatic synthesis of aspartame from N-formyl-L-aspartic acid and L- or D,L-phenylalanine methyl ester involving an enzymatic deformylation reaction simultaneously with an enzymatic coupling reaction, as well as to one-pot di- or oligopeptide synthesis by simultaneous enzymatic coupling and deformylation reactions in general. |
申请公布号 |
AU1898500(A) |
申请公布日期 |
2000.07.12 |
申请号 |
AU20000018985 |
申请日期 |
1999.12.20 |
申请人 |
HOLLAND SWEETENER COMPANY V.O.F. |
发明人 |
PETER JAN LEONARD MARIO QUAEDFLIEG;THEODORUS SONKE;ADOLF FRITZ VOLKER WAGNER |
分类号 |
C07K5/06;C07K5/072;C07K5/075;C12N9/80 |
主分类号 |
C07K5/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|