A tank handling apparatus which is usable for loading and unloading of a tank such as an oxygen tank with respect to a location of usage such as an emergency vehicle. The handling apparatus is capable of securely locking the tank in place at the storage or usage position while at the same time easily allowing it to be detached therefrom and transferred to a tank service position for removal and replacement or servicing. The apparatus includes a unique locking mechanism including a locking hook construction and a locking housing with a locking slide movably mounted therein wherein a locking handle is secured thereto for movement of the locking slide between the position of engagement and the position of release of the locking hook. Multiple locking hooks and locking slides can be utilized for further secure attachment of the tank in the storage position.