Optical characterization of nucleic acids and oligonucleotides
摘要
A method for the enzymatic sequencing of DNA is disclosed which uses dideoxythio-nucleotides or dideoxyamino-nucleotides as terminators that are labeled with one or two fluorescent dyes coupled to the terminators either before or after polymerization. The dye labeled DNA fragments are separated in a single lane of a separation system and then the terminal bases of each of the fragments are identified by measuring the fluorescent lifetime of the dye attached to the terminators.
申请公布号
US6087101(A)
申请公布日期
2000.07.11
申请号
US19970990734
申请日期
1997.12.15
申请人
GRUELICH, KARL OTTO;SEIDEL, CLAUS;WOLFRUM, JUERGEN;AUER, MANFRED;GAUTEL, MATTHIAS;GOODY, ROGER;LABEIT, SIEGFRIED
发明人
GRUELICH, KARL OTTO;SEIDEL, CLAUS;WOLFRUM, JUERGEN;AUER, MANFRED;GAUTEL, MATTHIAS;GOODY, ROGER;LABEIT, SIEGFRIED