| 摘要 |
PROBLEM TO BE SOLVED: To efficiently obtain an optically active 4-halo-3-hydroxybutyric acid ester having a high optical purity by carrying out the asymmetric reduction of a 4-haloacetoacetic acid ester with β-ketoacyl-acyl carrier protein reductase constituting type II fatty acid synthase. SOLUTION: The objective optically active (S)-4-halo-3-hydroxybutyric acid ester [e.g. ethyl (S)-4-chloro-3-hydroxybutyrate or the like] having a high optical purity is efficiently obtained by carrying out the asymmetric reduction of a 4-haloacetoacetic acid ester (e.g. ethyl 4-chloroacetoacetate or the like) or its derivative by the use of β-ketoacyl-acyl carrier protein reductase constituting type II fatty acid synthase which is composed of the protein containing the amino acid sequence represented by the formula or the protein containing the amino acid sequence in which one or more amino acids are added, deleted or substituted to the amino acid sequence represented by the formula. |
