| 摘要 |
A process is described for preparing transfectacons, or particles, of calciu m phosphate and a desired nucleic acid comprising admixing calcium divalent cation, phosphate multivalent anion, and the desired nucleic acid to form a precipitation mixture, wherein the precipitation mixture comprises an initia l phosphate anion concentration of about 0.2 to 0.5 mM; and incubating the precipitation mixture for about 10 to 60 minutes to form transfectacons comprising calcium phosphate and the desired nucleic acid. A process is also provided for delivering desired nucleic acid to eukaryotic tissue or cells comprising introducing to the tissue or cells the transfectacons so prepared . Additionally, a process is disclosed for introducing a desired nucleic acid into an eukaryotic host cell comprising the above two steps, followed by diluting the precipitation mixture and admixing it with an eukaryotic host cell lacking a cell wall to form a transfection mixture; and incubating the transfection mixture to allow the eukaryotic host cell to take up the transfectacons to form a transfected cell.
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