ANTAGONISTIC MICROORGANISM STRAIN FOR PREVENTING BLIGHT OF CROPS, MICROORGANISM PREPARATION COMPRISING THE SAME AND THE USE THEREOF

摘要

PURPOSE: Provided is a microorganism preparation containing antagonistic microorganisms for preventing plant blight which is caused by fungi. The microorganisms are Bacillus subtilis GB-0365(KFCC-11071) and Bacillus sp. GB-017(KFCC-11070) isolated from soil. CONSTITUTION: Soil is mixed with distilled water and autoclaved. The autoclaved solution is filtered with gauze and mixed with agar. After autoclaving, the mixture is kept at water bath at 50°C and mixed with a few drops of supernatant of soil and water mixture. The mixied agar is poured into a plate and overlaid with agar again. Potato agar medium containing spore of plant pathogen is poured onto over the solified agar. The antagonistic microorganisms are isolated from the three layered plate and confirmed antagonistic character by measuring clear zone of isolated microorganisms. The isolated microorganisms are identified as Bacillus subtilis GB-0536 (KFCC-11071) and Bacillus sp. GB-041 (KFCC-11070) which inhibit the growth of plant pathogens such as Botrytris cineria, Fusarium sp., Pythium sp., Pyhtophtora capsici, Alternaria citri, Cladosporium cucumerium, Colletotrichum gloeosporioides, Pleospora sp., Rhizoctonia solani.