发明名称 |
Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
摘要 |
Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments the methods involve co-expression of a prokaryotic disulfide isomerase, such as DsbC or DsbG and a gene encoding a recombinant eukaryotic polypeptide. Exemplary polypeptides disclosed include tissue plasminogen activator.
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申请公布号 |
US6083715(A) |
申请公布日期 |
2000.07.04 |
申请号 |
US19970871483 |
申请日期 |
1997.06.09 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM;GENENTECH, INC. |
发明人 |
GEORGIOU, GEORGE;OIU, JI;BESSETTE, PAUL;SWARTZ, JAMES |
分类号 |
C12N15/09;C07K14/47;C07K14/65;C07K14/81;C12N1/21;C12N9/02;C12N9/64;C12N9/72;C12N15/70;C12P21/02;(IPC1-7):C12P21/06;C12N1/12;C12N5/00;C07H21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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主权项 |
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地址 |
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