发明名称 |
Process for the recovery and purification of a recombinant protein from a cell |
摘要 |
The present invention involves a process of purifying and recovering a recombinant protein from a suspension of cells. The process of the present invention involves extracting a recombinant protein from a concentrated suspension of cells using a water-miscible organic solvent, isolating the recombinant protein from the suspension of cells, concentrating the recombinant protein to remove the water-miscible organic solvent, precipitating the recombinant protein using an acid, washing the recombinant protein with the salt or free form of a suitable organic acid and recovering the recombinant protein.
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申请公布号 |
US6080844(A) |
申请公布日期 |
2000.06.27 |
申请号 |
US19980065224 |
申请日期 |
1998.04.23 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
CARNEY, RONALD E.;ARNDT, JOSEPH;LIST, JULIE R.;SCHWARTZ, ELLEN MARIE |
分类号 |
C12N15/09;C07K1/36;C07K14/47;(IPC1-7):C07K1/14 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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