发明名称 |
Soluble T cell receptors |
摘要 |
An intact, assembled T cell receptor (TcR) in soluble form is provided. The soluble TcR is prepared by splicing the extracellular domains of a T cell receptor to the glycosyl phosphatidylinositol (GPI) membrane anchor sequences of Thy-1. The molecule is expressed in the absence of CD3 on the cell surface, and can be cleaved from the membrane by treatment with phosphatidylinositol specific phospholipase C (PI-PLC). The alpha and beta chains of the soluble molecule are paired in the native conformation as judged by reactivity with the anti-V beta 8 monoclonal antibody F23.1, and with the anti-clonotypic monoclonal antibody 1B2. The soluble TcR is a disulfide linked dimer with a molecular mass of 95 kDa on SDS-PAGE under nonreducing conditions, and 47 kDa after reduction.
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申请公布号 |
US6080840(A) |
申请公布日期 |
2000.06.27 |
申请号 |
US19950393157 |
申请日期 |
1995.02.17 |
申请人 |
SLANETZ, ALFRED E.;BOTHWELL, ALFRED L. M. |
发明人 |
SLANETZ, ALFRED E.;BOTHWELL, ALFRED L. M. |
分类号 |
C07K14/705;(IPC1-7):C07K14/00 |
主分类号 |
C07K14/705 |
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地址 |
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