发明名称 |
NUCLEOLUS AUTOANTIGENIC MARKER FOR SYSTEMATIC LUPUS ERYTHEMATOSUS |
摘要 |
A novel nucleolus protein has been identified and cloned using human autoimmune serum. Its cDNA and amino acid sequences have been determined and are disclosed. This antigenic protein, termed ASE-1 has an approximate molecular mass of 55 kDa. Immunoblot analysis indicates that both the native protein and the in vitro translation products of the cDNA migrate on SDS-PAGE at an apparent molecular mass of 90 kDa. Indirect immunofluorescence analysis using antibodies generated to cloned regions of ASE-1 indicates that this protein occurs at the fibrillar centers of the nucleolus in the putative sites of rDNA transcription. During cell division ASE-1 localizes to the nucleolus organizer regions of the chromosomes, where it is closely associated with RNA polymerase 1. As an autoantigenic nucleolar protein, ASE-1 has been found to be a reliable serum marker for systemic lupus erythematosus (SLE). This finding makes ASE-1 useful in the clinical detection and characterization of the disease. To identify the presence of SLE in an individual patient, a serum sample is taken and screened against the cloned ASE-1 protein to identify sera with anti-ASE-1 autoantibodies. This screening can be done using an ELISA assay, western blot techniques, or by binding the antigen to microspheres and identifying reactive sera by flow cytometry.
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申请公布号 |
WO0036087(A2) |
申请公布日期 |
2000.06.22 |
申请号 |
WO1999US29767 |
申请日期 |
1999.12.15 |
申请人 |
RATTNER, JEROME, B.;WHITEHEAD, CLARK |
发明人 |
RATTNER, JEROME, B.;WHITEHEAD, CLARK |
分类号 |
C07K14/47;C12N1/21;C12N15/12;(IPC1-7):C12N1/21;C12N5/10;C12N15/86;C12P21/00;C12N15/06;G01N33/564 |
主分类号 |
C07K14/47 |
代理机构 |
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地址 |
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