发明名称 |
RAFFINOSE SYNTHETASE GENE, PROCESS FOR PRODUCING RAFFINOSE, AND TRANSFORMED PLANT |
摘要 |
Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40 DEG C; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol. <IMAGE> |
申请公布号 |
EP0994186(A4) |
申请公布日期 |
2000.06.21 |
申请号 |
EP19970909634 |
申请日期 |
1997.10.24 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
OSUMI, CHIEKO;NOZAKI, JINSHI;KIDA, TAKAO |
分类号 |
C12N1/21;C12N9/10;C12N15/54;C12N15/82 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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地址 |
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