| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new peptide, a peptide having a specific amino acid sequence and having neurotransmission inhibitory action, useful as e.g. a preventive/therapeutic agent for cerebroneural diseases by isolation from the venom of a kind of scorpion, called CTX. SOLUTION: This new peptide is such one as to have an amino acid sequence of the formula and have neurotransmission inhibitory action, being useful as e.g. a preventive/therapeutic agent for cerebroneural diseases because of being effective in inhibiting neurotransmission. This new peptide is obtained by the following procedure: a dried product of CTX crude venom prepared from the venom of the above kind of scorpion is dissolved in water, the resulting aqueous solution is subjected to ion exchange chromatography to effect elution with varied pH or salt concentration of a buffer solution for elution; subsequently, the resulting elute is subjected to reversed-phase chromatography using a silica gel support modified with hydrophobic groups to effect elution through concentration gradient elution method using a hydrophilic solvent such as acetonitrile to carry out separation and purification. |
