| 摘要 |
The present invention relates to a protein having an activity of conferring on yeast brewer's yeast-type flocculating property; a DNA coding for the protein; a plasmid containing the DNA; a method for producing a yeast strain wherein brewer's yeast-type flocculating property has been conferred or enhanced with the use of the DNA and a method for producing a yeast strain wherein brewer's yeast-type flocculating property has been eliminated or reduced with the use of the DNA; and a method for eliminating or reducing brewer's yeast-type flocculating property of yeast by inhibiting the expression of the DNA. The present invention also relates to a yeast strain wherein brewer's yeast-type flocculating property has been conferred, enhanced, eliminated or reduced by the above method. Further, the present invention relates to a method for producing a brewed product comprising culturing the above yeast strain as well as a brewed product obtained by the method. According to.the present invention, there are provided the Lg-Flo1 protein having an activity of conferring on yeast brewer's yeast-type flocculating property and the DNA strand of Lg- FL01 coding for the protein. By introducing the above DNA of the invention into yeast cells as a foreign gene by genetic engineering techniques, i.e., introducing the DNA into yeast cells as an extranuclear gene and(/or) a nuclear gene, it is possible to confer on yeast brewer's yeast-type flocculating property or enhance this property in yeast. On the contrary, by introducing a DNA is obtained by destroying the DNA of the invention into yeast cells, or by inhibiting the expression of the DNA of the invention, it is possible to convert a flocculent yeast strain into a non-flocculent strain, or to reduce flocculating property.
|
