| 摘要 |
PROBLEM TO BE SOLVED: To detect the fluorescence from a fluorescent pigment bonded to a sample as a label without being influenced by Raman scattering or Rayleigh's scattering. SOLUTION: A sample is separated, and the separated component is successively sent to a part to be detected 2c. A laser beam of 600 nm or more from the laser beam source 12 of an optical measurement part 10 is emitted to the part to be detected 2c through a dichroic mirror 14 and a lens 16 to absorb multiple photons to a fluorescent pigment bonded to the separated component, and the fluorescent pigment is excited to generate fluorescence. The fluorescence is taken into the optical measurement part 10 to detect the fluorescence having a wavelength of 510 nm or less by a photomultiplier 20, the fluorescence having a wavelength more than 510 nm and not more than 560 nm by a photomultiplier 24, the fluorescence having a wavelength exceeding 560 nm and not more than 580 nm by a photomultiplier 28, and the fluorescence having a wavelength exceeding 580 nm by a photomultiplier 30, respectively. |
