ERA COMPLEXES AND USES THEREOF

摘要

Provided are Era/RNA complexes comprising at least one Era protein and at least one RNA species selected from 16S rRNA, 23S rRNA, 5S rRNA, or mRNA of Era, or an oligomeric fragment, probe, or primer derived from such 16S rRNA, 23S rRNA, 5S rRNA, or mRNA of Era. The formation of such complexes has been found to be necessary for high levels of GTPase activity of the Era protein, and viability of microorganisms in which such complexes are present. Also provided are methods for preparing Era/RNA complexes in vivo and in vitro, for identifying inhibitors of Era/RNA complex formation and complex stability, for identifying compounds that bind to Era proteins and Era/RNA complexes, and for identifying inhibitors of Era protein enzymatic and ligand binding activities in isolated Era proteins and Era/RNA complexes.