| 摘要 |
PURPOSE: Humanized antibody on surface antigen pre-S1 of hepatitis B virus (HBV) is provided which is derived from mouse monoclonal antibody (KR 127), The humanized antibody maintains the affinity on the antigen but decreases the antigenecity of mouse antibody on the human body by changing amino acid residues specific for mouse into human specific one. CONSTITUTION: Humanized variable region of heavy chain is derived from variable region of heavy chain of human antibody DP7 substituted into mouse framework residues and complementarity-determining region (CDR)1 and 3, and part of 2. Complete humanized heavy chain is constructed by joining leader sequence for secretion, constant region of heavy chain from pRC/CMV-HC-HuS, and humanized variable region. Humanized variable region of light chain is derived from variable region of light chain of human antibody DPK12 substituted into mouse framework residues and complementarity-determining region (CDR)1, 2, and 3. Complete humanized light chain is constructed by joining leader sequence for secretion, constant region of heavy chain from pKC-hr-HuS, and humanized variable region. Each complete chain is cloned into expression vector, pRc/CMV, and transfected into COS7 cell for the production of antibody.
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