PREPARING METHOD OF ROOT OF GINSENG FROM PLANT TISSUE CULTURE

摘要

PURPOSE: A method for producing Insam(ginseng) root by using plant tissue culture is provided, therefore it is possible to produce high content saponin and Insam(ginseng) extract regardless of weather, soil, or damage from disease and harmful insects. CONSTITUTION: The method for producing Insam(ginseng) root by using plant tissue culture comprises the steps of: (i) inoculating and cultivating germinated seed or the intercept of an undried Insam(ginseng) in an MS solid medium that contains growth hormone mixed of auxin and cytokinin at 25deg.C for some 4 weeks to induce callus; (ii) cultivating the induced callus in an MS solid medium for inducing Insam(ginseng) root which contains growth hormone mixed of auxin 2-4mg/L and cytokinin 0.5-1mg/L; (iii) cultivating the induced Insam(ginseng) root in an SH liquid medium which contains growth hormone mixed of auxin 2-4mg/L and cytokinin 0.5-1mg/L; and (iv) cultivating in an SH liquid medium which contains only auxin 2-4mg/L.