| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new polynucleotide which contains a polynucleotide sequence coding for a polypeptides of LD78 gene group or its complementary polypeptides and is useful in the estimate of the progressive degree of HIV infection or the diagnosis, treatment or the like for AIDS, cancer or the like. SOLUTION: This polynucleotide is a purified new polynucleotide containing a polynucleotide sequence coding for the polypeptides of LD78 gene group or its complementary polypeptides and can estimate the disease progressive degree on HIV infectious disease by analyzing the number of chemokine LD78 gene group by means of PCR method and carry out the treatment for HIV infection, e.g. AIDS or the like, and the treatment or the like for cancer by using the polypeptides of this gene group. This purified polynucleotide is obtained by treating the DNA extracted from human peripheral blood lymphocytes with a primer comprising of the partial sequence of LD78 gene by means of PCR method, treating it with a restriction enzyme and separating and purifying the resultant fragments by agarose electrophoresis. When treated with the restriction enzyme, the LD78 B group is cleaved, but the LD78 A group is not cleaved. |
