摘要 |
PROBLEM TO BE SOLVED: To obtain a new protein carrier molecule which is derived from a cellulose-binding protein having plural repeats of an enzyme-binding domain showing a binding ability to a carrier molecule-binding domain of cellulase, and gives highly active enzyme complexes such as immobilized enzyme. SOLUTION: The new protein carrier molecule, which is structurally derived from a cellulose-binding protein having plural repeats of an enzyme-binding domain (C) showing a binding ability to a carrier molecule-binding domain (D) of cellulase, contains intact a cellulose-binding domain(CBD) which the cellulose-binding protein originally has, or has the structure in which the cellulose-binding domain was excluded and each of the plural number of enzyme- binding domains (C) was substituted for a plural kinds of enzyme-binding domains (C1, C2, and so on) having mutually different binding specificities, and can be used for producing highly active enzyme complexes such as immobilized enzyme and so on, can be obtained by linking a cellulose-binding protein gene derived from C. josui to cohesin gene for expression. |